ABSTRACT
OBJECTIVE: To study the effect of different media on seed germination and young seedling propagation of Anoectochilus roxburghii. METHODS: A. roxburghii seeds were cultured in 6 basal media for 12 weeks, and germination rates were calculated; orthogonal design was used to study the effects of NAA and 6-BA on the propagation of young seedlings of A. roxburghii. After 4 months, the leaf number, stem internode number, root number, the length of root, plant height, and fresh mass were measured respectively. RESULTS: The effects of different media on seed germination and protocorm differentiation of A. roxburghii were different. Germination rate was the highest on MS medium, which was 60.38%, and protocorm differentiation was optimal on half-strength MS medium. Different combinations of NAA and 6-BA didn't show significant difference on the growth of young seedlings of A. roxburghii, and the appropriate medium was 1/2MS + NAA 1.0 g · L-1 + 6-BA 2.0 mg · L-1. CONCLUSION: The optimization of culture medium can stimulate seed germination and young seedling propagation of A. roxburghii. Copyright 2012 by the Chinese Pharmaceutical Association.